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. 2015 Mar 24;10(3):e0120860. doi: 10.1371/journal.pone.0120860

Fig 6. Determination of unsaturated disaccharide components in IdoA-rich fractions of different molecular weights.

Fig 6

(A) Separation of IdoA-rich domain and partially degraded samples by ChaseACII. One mg of samples were subjected partial digestion with 1 unit of ChaseACII at 37°C for 1 h and then resulting degraded samples were fractionated by an HPSEC systems with an Asahipak 510HQ column (7.6 mm, i.d. × 300 mm) as described previously [27]. The fraction consisting of oligosaccharides contained an IdoA-rich domain as the GlcA-rich domains were degraded by ChaseACII. (B) Separation of IdoA-rich domain at the different molecular weight. Samples (0.1 mg) after digestion with ChaseACII were subjected by gradient SDS-PAGE (PAGEL NPG-1020L, 10–20%). The electrophoresis was performed as described previously [27]. (C) Determination of unsaturated disaccharides in IdoA-rich domain at the different molecular weight. (D) Determination of unsaturated disaccharides in fraction b, c and d in Fig. 6A. Peaks: 1, ΔDi-0S; 2, ΔDi-4S; 3, ΔDi-6S; 4, ΔDiUA-2S; 5, ΔDi-diSE; 6, ΔDi-diSB; 7, ΔDi-diSD.