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. 2015 Mar 12;15:30. doi: 10.1186/s12935-015-0176-2

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© Uchino et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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After treatment with 100 nM ATRA with or without 320 nM ruxolitinib for 5 days, HT93A cells were stimulated with 50 ng/mL G-CSF for 60 min. Expression of phosphorylated STAT5 was evaluated by intracellular staining and analyzed by flow cytometry. Ruxolitinib-treated HT93A cells showed significantly lower phosphorylated STAT5 expression. Histograms (A) and bar graphs (B) represent phosphorylated STAT5 expression. Experiments were independently repeated three times and results are shown as mean ± SD.