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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: J Am Chem Soc. 2015 Feb 11;137(7):2432–2435. doi: 10.1021/ja511833y

Figure 2.

Figure 2

(A) Workflow of the capture and enrichment strategy using the functionalized NPs. (B) SDS-PAGE analysis with Sypro Ruby (top) and Pro-Q Diamond (bottom) staining confirmed the highly specific enrichment of phosphoproteins (β-casein and pepsin) from standard protein mixtures containing non-phosphoprotein (BSA). M, molecular markers; LM, loading mixture before enrichment; FT, flow-through; and E, elution after enrichment. (C–D) Global enrichment of phosphoproteins from (C) HEK 293 cell lysate (equal amount loading, 15 µg) and (D) swine heart tissue extract (equal amount loading, 10 µg) as demonstrated by SDS-PAGE analysis with Sypro Ruby (left side) and Pro-Q Diamond staining (right side).