Skip to main content
. 2015 Mar 24;10(3):e0120578. doi: 10.1371/journal.pone.0120578

Fig 1. Measurement of NEU3 and EGFR levels in NEU3- and/or EGFR- transfected NIH-3T3 cells.

Fig 1

(A) NEU3 and EGFR protein levels were analyzed by western blotting using antibodies for NEU3 and EGFR, respectively. The proteins were hardly detectable in the cells without the transfection of NEU3 and EGFR genes. Relative mRNA levels for murine EGFR/GAPDH were 0.7–0.9 in the controls and all the clones generated, and those for human EGFR/GAPDH were 0.1–0.2 in controls and NEU3-cells and 5,000–6,000 in the EGFR- and EGFR/NEU3-cells. (B) The sialidase activity levels toward mixed gangliosides (Bovine brain, Sigma) as a substrate were estimated. Only a slight sialidase activity was shown in vector controls and in EGFR-transfected cells.