Fig 9. Internalization of FM4–64 is delayed in mini_cnx1 cells under nitrogen starvation.

Time course of FM4–64 uptake analyzed by fluorescence microscopy for the WT and mini_cnx1 strains. Cells were grown in EMM to mid-logarithmic phase or in EMM-N medium to induce nitrogen starvation for 2 days and stained with FM4–64 to monitor the endocytic flux. The rate of endocytosis was followed by fluorsence microscopy with FM4–64, and cell images were recorded at the indicated times.