| Tissue | 1. | Femurs and tibias |
| Supplies | 2. | Scissors |
| 3. | 50ml conical tube | |
| 4. | PBS | |
| 5. | Complete expansion media (CEM) | |
| 6. | 25G 1″ needle | |
| 7. | 5ml disposable syringe with Leur lock tip | |
| 8. | 70μm mesh strainer | |
| 9. | Trypan blue | |
| 10. | Microcentrifuge tubes | |
| 11. | Hemocytometer | |
| 12. | Micropipettes and pipettes with tips | |
| 13. | 140×20mm cell culture dishes | |
| Equipment | 14. | Large centrifuge |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.