| Tissue | 1. | Subcutaneous/inguinal fat tissue |
| Supplies | 2. | Scissors |
| 3. | Scale with measurements in grams | |
| 4. | 50ml conical tubes | |
| 5. | PBS | |
| 6. | 265units/mg Collagenase type-1 | |
| 7. | Bovine serum albumin (BSA) | |
| 8. | 2mM Calcium chloride in ddH2O (CaCl2) | |
| 9. | 70μm mesh strainer (optional) | |
| 10. | Parafilm | |
| 11. | Complete expansion media (CEM) | |
| 12. | 50ml Steriflip disposable vacuum filtration system with 0.22μm filter | |
| 13. | Vacuum tubing | |
| 14. | Timer | |
| 15. | Wedge-shaped object | |
| 16. | Trypan blue | |
| 17. | Microcentrifuge tubes | |
| 18. | Hemocytometer | |
| 19. | Micropipettes and pipettes with tips | |
| 20. | T225 cell culture flasks | |
| Equipment | 21. | Incubator shaker |
| 22. | Large centrifuge |
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