Supplies	1.	Phosphate buffered saline (PBS)
	2.	Hank's balanced salt solution (HBSS)
	3.	Dulbecco's Minimum Essential Media: Nutrient Mixture F-12 (DMEM/F-12 media)
	4.	Fetal bovine serum (FBS)
	5.	2mM L-glutamine
	6.	10,000units/ml Penicillin – 10,000μg/ml streptomycin (Pen-Strep)
	7.	140×20mm cell culture dishes
	8.	T225 cell culture flasks
	9.	0.25% Trypsin-EDTA
	10.	Trypan blue
	11.	Microcentrifuge tubes
	12.	Cryovials
	13.	Dimethylsulfoxide (DMSO)
	14.	Stericup vacuum-mediated 0.22μm filter unit
	15.	1ml Leur lock disposable syringes with 0.1ml graduations
	16.	27G ½″ needle
	17.	Micropipettes and pipettes with tips
	18.	Hemocytometer
	19.	Aspirator
Equipment	20.	Water bath
	21.	Large centrifuge
	22.	CO2 Incubator
	23.	Biosafety hood
	24.	Liquid nitrogen dewar