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. 2015 Mar 24;10(3):e0120648. doi: 10.1371/journal.pone.0120648

Fig 1. Identification of gp120/CD4 blocking antibodies.

Fig 1

The presence of antibodies with the capacity to block the interaction between CD4 and gp120 was evaluated with a flow cytometry competitive assay using a chronically infected cell line and a huCD4mIgG recombinant protein. A) MOLT-NL4.3 (black line) and uninfected-MOLT cells (gray) were stained with huCD4mIgG (10μg/mL) and a DyLight-649 Fab2 Goat anti-mouse IgG as secondary antibody. Dotted line shows the staining of MOLT-NL43 cell using a mouse IgG1 isotype control. B) MOLT-NL4.3 cells were stained with serial dilutions of huCD4mIgG. C) IgGb12 inhibition of the binding of huCD4mIgG to MOLT-NL4.3.