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. 2015 Mar 24;10(3):e0120648. doi: 10.1371/journal.pone.0120648

Fig 2. Gp120/CD4 blocking antibodies in ART-naive HIV-1 infected patients.

Fig 2

A) The presence of gp120/CD4 blocking antibodies was determined in the plasma of 35 ART-naive HIV-1 infected patients (squares) and compared with 10 uninfected healthy control individuals (HC, triangles). The cytometric competitive assay was performed using the huCD4mIgG recombinant protein and the MOLT-BaL cell line. Differences were calculated using Mann-Whitney U test. Dotted line shows cut-off of positivity calculated as mean+2xSD of HC samples. B) Correlation between levels of gp120/CD4 blocking antibodies, using MOLT-BaL and MOLT-NL4.3 cells, was determined by Spearman´s test. C) Gp120/CD4 blocking antibodies were determined in two plasma samples separated by one year from the same HIV-1 infected individuals (paired t-test). Dotted line shows cut-off of positivity as in panel A. D and E) Correlation analysis between the percentage of gp120/CD4 blocking activity of plasma samples and time after diagnosis or viral load (VL) was performed using Spearman´s correlation.