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. 2015 Mar 24;10(3):e0120648. doi: 10.1371/journal.pone.0120648

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© 2015 Carrillo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — A) The IgG response against Env was evaluated by flow cytometry using MOLT-BaL and uninfected-MOLT cell line. The levels of anti-Env IgG antibodies were calculated as MFI ratio (MOLT-BaL/MOLT). B) Titer of antibodies against the soluble trimer BG505 SOSIP.664 gp140 was determined by ELISA. A correlation analysis between the anti-Env levels and gp120/CD4 blocking activity is shown (Spearman´s correlation). C) A set of anti-Env antibodies, whose reactivity is well-known, was used to evaluate the specificity of the antibodies that were able to block the binding of the huCD4mIgG recombinant protein to MOLT-BaL cells. The antibodies tested included CD4bs Abs (VRC01, VRC03), anti-glycosylated epitopes (2G12, PGT126), anti-glycosylated-quaternary epitopes (PG9, PG16), CD4 induced epitopes (17b,48d), anti-gp41 (2F5, 4E10) and a polyclonal goat anti-gp120 (anti-gp120) obtained by immunization using rgp120.