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. 2015 Mar 24;10(3):e0121570. doi: 10.1371/journal.pone.0121570

Fig 1. Generation of tg mice expressing the mutant 3R Tau under the mThy-1 promoter.

Fig 1

A. Diagram of the mThy-1 construct with the mutant 3R Tau. B. Levels of human 3R Tau and C murine Tau mRNA expressed as a ratio to the housekeeping gene GAPDH in non-tg and 3R Tau Lines 2, and 13. D. Representative Western blot (SDS) and analysis of the levels of E Total Tau and F 3R Tau with the insoluble (membrane) fractions showing that Line 13 was a higher expresser of total Tau and 3R Tau compared to Line 2 and non-tg mice. Across all lines Tau was detected as double bands between 50–60 kDa. In the Pick’s Disease cases 3R Tau co-migrated at a similar level as in the 3R Tau tg mice. For analysis, N = 6 non-tg and N = 6 mThy-1 3R Tau tg mice (3–4 months old) from each line were utilized. * = P < 0.05 when compared to non-tg control using one way ANOVA with Dunnett’s posthoc test. # = P < 0.05 when comparing Line 2 and 13 using one way ANOVA with Fisher’s posthoc test.