Table 2. Sedimentation velocities of antibodies 1 and 2 measured at 40 krpm.
| antibody 1: 73.5 g/L | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| run | f/f0 | rmsd | signal | signal* | dn/dc* | LMW | monomer | dimer | oligomers | ||
| [ΔΦ] | [ΔΦ] | [ΔΦ] | [mL/g] | [%] | [S] | [%] | [S] | [%] | [%] | ||
| 4/2 | 14.91 | 0.703 | 33.18 | 32.58 | 0.195 | 1.8 | 3.12 | 97.1 | 3.87 | 1.5 | 1.4 |
| 4/3 | 15.15 | 0.762 | 33.69 | 32.98 | 0.197 | 2.1 | 3.15 | 96.0 | 3.84 | 2.3 | 1.7 |
| 4/6 | 15.24 | 0.719 | 33.82 | 33.16 | 0.199 | 2.0 | 3.15 | 96.2 | 3.84 | 2.0 | 1.8 |
| 4/7 | 15.52 | 0.706 | 34.51 | 33.27 | 0.199 | 3.6 | 3.14 | 95.9 | 3.76 | 2.1 | 2.0 |
| m | 15.21 | 0.723 | 33.80 | 33.00 | 0.198 | 2.4 | 3.14 | 96.3 | 3.83 | 2.0 | 1.7 |
| SD | 0.25 | 0.027 | 0.55 | 0.30 | 0.002 | 0.8 | 0.01 | 0.5 | 0.05 | 0.3 | 0.3 |
| antibody 1: 147 g/L | |||||||||||
| run | f/f0 | rmsd | signal | signal* | dn/dc* | LMW | monomer | dimer | oligomers | ||
| [ΔΦ] | [ΔΦ] | [ΔΦ] | [mL/g] | [%] | [S] | [%] | [S] | [%] | [%] | ||
| 5/1 | 35.54 | 0.747 | 36.51 | 35.29 | 0.106 | 3.3 | 1.35 | 87.8 | 1.67 | 3.3 | 9.0 |
| 5/3 | 40.02 | 0.883 | 37.75 | 36.00 | 0.108 | 4.6 | 1.32 | 86.9 | 1.57 | 3.7 | 9.5 |
| 5/4 | 38.20 | 0.700 | 36.35 | 35.00 | 0.105 | 3.7 | 1.33 | 83.9 | 1.57 | 4.7 | 11.4 |
| 5/7 | 41.28 | 0.737 | 34.75 | 34.12 | 0.102 | 1.8 | 1.31 | 86.6 | 1.55 | 4.3 | 9.1 |
| m | 38.76 | 0.767 | 36.34 | 35.10 | 0.105 | 3.4 | 1.33 | 86.3 | 1.59 | 4.0 | 9.7 |
| SD | 2.49 | 0.080 | 1.23 | 0.78 | 0.003 | 1.2 | 0.02 | 1.7 | 0.05 | 0.6 | 1.2 |
| antibody 2: 51 g/L | |||||||||||
| run | f/f0 | rmsd | signal | signal* | dn/dc* | LMW | monomer | dimer | oligomers | ||
| [ΔΦ] | [ΔΦ] | [ΔΦ] | [mL/g] | [%] | [S] | [%] | [S] | [%] | [%] | ||
| 2/1 | 8.05 | 0.444 | 24.67 | 23.28 | 0.201 | 6.0 | 3.35 | 98.7 | 4.72 | 1.2 | 0.1 |
| 2/4 | 8.00 | 0.442 | 24.05 | 22.77 | 0.196 | 5.6 | 3.35 | 98.9 | 4.81 | 1.0 | 0.1 |
| 2/5 | 7.74 | 0.431 | 23.69 | 22.92 | 0.198 | 3.3 | 3.41 | 98.3 | 4.71 | 1.6 | 0.1 |
| 2/7 | 8.08 | 0.451 | 24.15 | 22.78 | 0.197 | 6.0 | 3.35 | 98.9 | 4.75 | 1.0 | 0.1 |
| m | 7.96 | 0.442 | 24.14 | 22.94 | 0.198 | 5.2 | 3.37 | 98.7 | 4.75 | 1.2 | 0.1 |
| SD | 0.16 | 0.008 | 0.40 | 0.24 | 0.002 | 1.3 | 0.03 | 0.3 | 0.04 | 0.3 | 0.0 |
| antibody 2: 102 g/L | |||||||||||
| run | f/f0 | rmsd | signal | signal* | dn/dc* | LMW | monomer | dimer | oligomers | ||
| [ΔΦ] | [ΔΦ] | [ΔΦ] | [mL/g] | [%] | [S] | [%] | [S] | [%] | [%] | ||
| 3/2 | 17.75 | 0.873 | 35.72 | 35.71 | 0.154 | 0.0 | 2.00 | 93.7 | 2.52 | 2.9 | 3.4 |
| 3/4 | 17.69 | 0.884 | 35.56 | 35.57 | 0.153 | 0.0 | 2.02 | 94.3 | 2.61 | 2.3 | 3.4 |
| 3/7 | 16.69 | 0.876 | 35.02 | 35.02 | 0.151 | 0.0 | 2.04 | 95.1 | 2.64 | 2.1 | 2.8 |
| m | 17.37 | 0.878 | 35.43 | 35.43 | 0.153 | 0.0 | 2.02 | 94.4 | 2.59 | 2.4 | 3.2 |
| SD | 0.60 | 0.006 | 0.37 | 0.36 | 0.002 | 0.0 | 0.02 | 0.7 | 0.06 | 0.4 | 0.3 |
Total signal and rmsd are given in units of fringe displacement. The refractive index increment dn/dc* is calculated from the total signal after subtraction of non-proteinaceous LMW material as an indication of mass retrieval. Relative contents of monomer and dimer+oligomers are calculated excluding the signal contribution of LMW material.