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. 2015 Mar 24;10(3):e0121484. doi: 10.1371/journal.pone.0121484

Fig 5. Effects of the pcs mutation and arm-variant overexpression on ring canal development.

Fig 5

(A—F) Stage 1-egg chambers were stained with antibodies against pY142 (A and D), pY654 (B and E) or Btk29A (C and F) in the wild-type (WT; A—C) and pcs gs mutant (D—F) ovaries. (G—K) Effects of overexpression of Y150F+Y667F (G), Y150F (H), Y667F (I), Y150E+Y667E (J), or arm + (K) on ring canal growth. Scale bars: 10 μm. (L) Quantitative comparisons of the ring canal diameter at stage 1 among different genotypes: wild type (n = 208), Y150F+Y667F (n = 166), Y150F (n = 181), Y667F (n = 160), Y150E+Y667E (n = 255), and arm + (n = 127). The means ± standard errors of ring canal diameters (in μm) at stage 1 are shown for the respective genotypes. The numbers of ring canals examined are shown in parentheses. Statistical differences between the indicated pairs were evaluated by Student’s t-test (**p<0.01; n.s., nonsignificant).