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. 2015 Mar 24;10(3):e0122753. doi: 10.1371/journal.pone.0122753

Fig 5. Autophagy regulates Sp clearance in A549 cells.

Fig 5

(A) A549 cells were transfected with RFP-plasmid for 24 h and then infected with Sp-GFP for 1 h (MOI = 30:1). Before infection, the cells were also treated with rapamycin (1 mM, 10 h), wortmannin (2 μM, 2 h), PI3K-I inhibitor, or ROS inhibitor. The fluorescence images are displayed to confirm the internalization of bacteria. (B) Number of internalized bacteria per cell. CFU experiments were utilized to count the invading bacteria inside the cells. The data are representative of 100 cells (one-way ANOVA; Tukey’s posthoc test). Data are representative of three experiments with similar results. Rapa: rapamycin; WT: wortmannin; Sp: S. pneumoniae; ROS: reactive oxygen species; Sp-GFP: Sp + pMV158GFP plasmid; CFU: colony-forming unit.