Fig 7. Effect of ScExlx1 on the enzymatic hydrolysis of cellulose.

Mercerized cotton fibers (1 mg) were incubated with 20 μg of ScExlx1, 20 μg of BSA or sodium acetate buffer (pH 5) for 72 h at 25°C. After incubation, temperature was raised to 50°C and cellulase cocktail from T. reesei was added (0.25 U) in a 3 h experiment. Reducing sugars were quantified by DNS method and compared with a glucose standard curve. Experiments were performed in triplicate, and the data points and error bars indicate means ± standard deviations. *Statistical differences between treatments (p<0.05) at each point.