Fig 8. ScExlx1 is a chitin active protein that enhances chitin hydrolysis.

Chitin from shrimp shells (5 mg) was incubated with 50 and 100 μg of ScExlx1, 50 μg of BSA, or sodium phosphate buffer 100 mM pH 7 for 24 h at 25°C. After incubation, temperature was increased to 37°C and chitinase from S. griseus (0.25 U) was added. After 2 hours of incubation, released N-acetylglucosamine was measured by DNS method and compared with a standard curve. Experiments were performed in triplicate, and the data points and error bars indicate means ± standard deviations. Letters indicate statistical differences in each treatment (p<0.05).