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. 2015 Mar 24;10(3):e0122452. doi: 10.1371/journal.pone.0122452

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© 2015 He et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Sal I and BglII restriction enzyme digestion analysis. M: 1kb DNA ladder; lane 1: pEGFP-hTfR, lane 2: pGEM-T-hTfR, lane 3: pEGFP-C1. (B) Immunoblot analysis of hTfR expression in cells. Cell lysates were probed by mouse anti-human TfR mAb. Left: representative WB picture of 4 separate experiments was shown. Right: Densitometric analysis of hTfR levels of the western blots. P values were calculated on the basis of Dunnett-t test (NS, not significant). (C) EGFP expression in CHO cells was detected by FCM and fluorescence microscope (insert).