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. 2015 Mar 24;10(3):e0120283. doi: 10.1371/journal.pone.0120283

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© 2015 Aerts et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (a) LLC-MK2 monolayers in 24 well-plates were infected with rHMPV at an MOI of 0.01 in quadruplicate. On days 2 through 4 pi, pictures were taken using fluorescent microscopy in 3 random fields (20x magnification) per well and the number of nuclei per GFP-expressing cell was calculated. ***, p < 0.001 comparing all other strains to rC-85473 and °°°, p < 0.001 comparing all other strains to rCAN98–75 using Repeated Measures Two-way ANOVA. (b) An example of the observed difference in syncytium formation between the 4 recombinant strains on day 3 pi.