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. 2015 Mar 24;10(3):e0120851. doi: 10.1371/journal.pone.0120851

Fig 6. STP mice displayed molecular signatures of human chronic pancreatitis and desmoplasia.

Fig 6

Significantly elevated Muc6 and Col1a1 expressions were detected in the STP mice. (A) IHC analysis of Muc6 expression in the pancreata from MT-TGFα (panel i) and STP mice (panel ii) treated with zinc sulfate for 8 months. Elevated expression of Muc6 was detected in evolving ADM and PanIN lesions of the STP mice. Magnification panels i and ii, 200X. (B) The RNA expressions of chronic pancreatitis marker Muc 6 and desmoplasia markers Ctgf (Connective tissue growth factor-1) and Col1a1 (Collagen type XXI alpha 1) in the S4, MT-TGFα, and STP mice were measured by qRT-PCR. The graphs present the relative expression of each gene to the S4 mice. Muc6 and Col1a1 were significantly upregulated in the STP mice compared to the MT-TGFα mice. Values are presented as mean ±SEM; Student’s t-test was employed, * p < 0.05; **p > 0.01; *** P < 0.005. (C) Elevated Col1a1 protein expression in the STP mice was confirmed by Western blot analysis of the total protein extracts from the pancreata of the three different models.