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. 2015 Mar 24;10(3):e0120321. doi: 10.1371/journal.pone.0120321

Fig 3. MMP-2 is a direct target of miR-519d-3p.

Fig 3

(A) Schematic illustrating construction of the luciferase reporter constructs (MMP-2 UTR and MMP-2 Mut UTR). (B) Luciferase assay of HTR8/SVneo cells co-transfected with miR-519d-3p mimics (or negative control) and the wild-type MMP-2 3`UTR reporter construct (MMP-2 UTR) or mutated MMP-2 3`UTR reporter construct (Mut UTR). (C, D) Quantitative real-time PCR and Western blot analysis of endogenous MMP-2 mRNA (C) and protein (D) expression in HTR8/SVneo cells transfected with miR-519d-3p mimic or miR-519d-3p inhibitor; data is expressed relative to the corresponding control cells (NC or NC inhibitor), P < 0.05.