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. 2015 Mar 10;5:191–197. doi: 10.1016/j.fob.2015.03.003

Fig. 2.

Fig. 2

Genotyping of offspring. (A) Schematic of Flanking Primer PCR assay. The primers bind on the flanking sequences. Insertion of the JT15-Spacer-Lox2272 sites adds 78 bases at the target site that increases the PCR band size from 419 bp (wild type) to 497 bp (mutant). Some samples show incorrect sized mutant band (see text for details). (B) Internal + External primer PCR. Genotyping PCR with a forward primer in the repair oligo region and a reverse primer downstream of Cr4 site. Insertion at Cr2 and Cr4 sites will yield bands of 410 and 225 base pairs respectively. See text for discussion on results for samples 6 and 53. M: 100 base pair Marker.