Table 1.
Summary of ligand binding assay of CXCR1 WT and mutants.1
| CXCR1 Residue | Location (Baldwin#) | CXCR1 and Mutants | Bmax (% of WT) | Kd (M) |
|---|---|---|---|---|
| WT | 100 ± 0 | 5.550e−09 (4.401e−009 to 7.000e−009) | ||
| Ser 132 | TM3, residue 47 | S132A | 179.6 ± 27.7 | 4.154e−09 (3.523e−009 to 4.897e−009) |
| Asp 134 | TM3, residue 49 | D134 N | 18.8 ± 2.0 | N.D. |
| D134V | 19.5 ± 0.5 | N.D. | ||
| Met 241 | TM6, residue 34 | M241V | 160.8 ± 13.3 | 1.531e−09 (1.068e−009 to 2.194e−009) |
| Phe 251 | TM6, residue 44 | F251A | 30.2 ± 5.2 | 1.026e−09 (8.500e−010 to 1.238e−009) |
| F251H | 256.9 ± 0.8 | 4.860e−09 (4.434e−009 to 5.328e−009) | ||
| F251Y | 213.0 ± 6.9 | 3.478e−09 (2.816e−009 to 4.294e−009) |
1
Bmax and Kd value were estimated from COS-7 cells ligand binding assay as described under “Section 4”. All assays were done in triplicates. Values are mean ± SEM from 3 experiments.