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. 2015 Mar 23;17(3):317–328. doi: 10.1016/j.neo.2015.02.003

Figure 3.

Figure 3

TGF-β downregulates Deptor expression through a Smad3-dependent pathway. (A) NMuMG cells were treated with TGF-β1 (5 ng/ml) as indicated and subsequently subjected to anti-ERα immunoblot analysis. Immunoblots are representative of three independent experiments. TGF-β1 (5 ng/ml for 48 hours) downregulates the expression of Deptor mRNA (B) and protein (C) in NMuMG cells. Data are mean (± SE; *P < .007). NMuMG (D) and 4T1 (E) cells were stimulated with TGF-β1 for 48 hours in the absence or presence of the p38 MAPK inhibitor, SB203580 (p38; 10 μM), or the Smad3 inhibitor, SIS3 (10 μM). Data are representative of three independent experiments. (F) 4T1 cells were cultured in the absence or presence of the TβR-I antagonist TβR-I type II inhibitor (10 μg/ml) or SB431452 (SB; 10 μM). Immunoblots are representative of three independent experiments. (G) MDA-MB-468 cells were stimulated with TGF-β1 (5 ng/ml) for 4 days. Immunoblots are representative of three independent experiments.