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. 2015 Mar 23;17(3):317–328. doi: 10.1016/j.neo.2015.02.003

Figure 6.

Figure 6

Deptor expression is necessary for pulmonary outgrowth of metastatic breast cancers. (A) ERα-negative breast cancer patient survival is significantly longer if Deptor expression is low as compared to tumors housing high Deptor expression. (B) Monitoring changes in AKT and S6K expression and activation in MDA-MB-231 cells engineered to overexpress Deptor. (C) Parental (MT) or Deptor-expressing MDA-MB-231 cells were propagated in 3D organotypic cultures for 6 days, during which time organoid growth was monitored longitudinally by bioluminescence on the indicated days. Data are the mean (± SE; n = 3; *P < .03). (D) Parental (MT) or Deptor-expressing MDA-MB-231 cells were injected into the lateral tail vein of nude mice. Pulmonary tumor growth was monitored by longitudinal bioluminescence imaging at the indicated times (n = 5; *P < .05). (E) Gross anatomy and H&E staining of lungs bearing parental (MT) or Deptor-expressing MDA-MB-231 tumors. (F) Immunohistochemical staining of Deptor in parental (MT) and Deptor-expressing MDA-MB-231 pulmonary lesions.