Fig. 1.

Plasmid pPOP15.9 found in P. popilliae ATCC 14706^T. Similarity of scaffold 8 to pBP68 and pBP614 (A). Open triangles indicate primers for gap enclosure. Restriction nuclease sites are shown as P (PstI) and X (XbaI). The numbers on the bold line for scaffold 8 represent DNA fragment length (kb). PCR was performed for gap closure of scaffold 8 (B). Plasmid was extracted and digested with PstI, XbaI, or both (C). Seventeen open reading frames were predicted. The inner circle represents the restriction map.