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. 2015 Mar 20;4:29–44. doi: 10.1016/j.mgene.2015.03.001

Fig. 4.

Fig. 4

Effect of pH on homomultimer formation of His-tagged Orf8 and Orf16 proteins. The recombinant proteins were incubated at various pH values and cross-linked with glutaraldehyde. The samples (250 ng/lane) were electrophoresed and CBB stained (A). The recombinant proteins were incubated at pH 2.5 and pH 7.5. After ultrafiltration (100-kDa cutoff), permeate (P) and retentate (R) fractions were electrophoresed and CBB stained (B). To ascertain pH stability, the recombinant proteins were incubated at various pH values, diluted, and shifted to pH 7–8. After cross-linking, the samples (25 ng/lane) were electrophoresed and silver stained (C). In all panels, large closed and open triangles indicate multimers and monomers, respectively.