Unrooted phylogenetic tree based on amino acid sequences of Orf8, Orf16, and other KfrA proteins. Abbreviations of class names (AC, Actinobacteria; BA, Bacilli; AP, Alphaproteobacteria; BP, Betaproteobacteria; GP, Gammaproteobacteria; DP, Deltaproteobacteria; CY, Cyanophyceae; NI, Nitrospira) are shown in bold letters with the exception of RK2. Gram-positive bacteria are indicated in larger letters.