Figure 1. Phylogenetic characterization of the hemagglutinin genes of Canadian HPAI H5N2 virus isolates.

The complete hemagglutinin (HA) gene sequences of the Canadian HPAI H5N2 viruses were aligned with H5 genes obtained from NCBI and GISAID EpiFlu™ databases using Clustal W. The supplementary file contains additional phylogenetic trees for the remaining gene segments and a Table that list and acknowledges the sequences included from GISAID. Phylogenetic and molecular evolutionary analysis was conducted using MEGA version 6 and the Maximum Likelihood method based on the Tamura Nei model²⁸. The tree with the highest log likelihood is shown. Bootstrap test involved 500 replicates to determine reliability of the inferred tree with only bootstrap values above 70% shown. Black upright triangles, Canadian HPAI H5N2; inverted triangle single Canadian human case of HPAI H5N1. Clade designations are based on WHO/OIE/FAO H5N1 Evolution Working Group nomenclature (http://www.who.int/influenza/gisrs_laboratory/h5_nomenclature_clade2344/en/).