FIG. 4.

SEB exposure leads to dysregulation of microRNA. Seventy-two hours after SEB exposure, lungs were perfused with heparinized PBS and were homogenized. After washing with sterile PBS, the mononuclear cell layer was isolated by density gradient centrifugation and total RNA was isolated. A, A heatmap depicting the differential expression of miRNA in the lungs of SEB exposed mice compared with vehicle. The accompanying color scape depicts mean expression values that are overexpressed (red) or underexpressed (green) above or below the mean, respectively. B, Fold change distribution plot of 1111 mouse-specific miRNA indicating several unchanged, upregulated, or downregulated miRNA. C, A proportional Venn diagram showing the number of miRNA that are overexpressed, underexpressed (<2-fold), or unchanged after SEB exposure.