FIG. 5.

Experimental validation of Top overexpressed and underexpressed miRNA. Seventy-two hours after SEB exposure, lungs were perfused with heparinized PBS and were homogenized. After washing with sterile PBS, the mononuclear cell layer was isolated by density gradient centrifugation and total RNA was isolated. A, q-RT PCR validation of the IPA generated Top overexpressed miRNA. B, q-RT PCR validation of the IPA generated Top underexpressed miRNA. Snord96a was used as the small endogenous control and expression of miRNA was normalized to vehicle. Data are represented as mean ± SEM from replicate samples. Lung infiltrating mononuclear cells were pooled from 5 mice in each group. Statistical significance is indicated as follows: ^*P < 0.05, ^***P < 0.001, ^****P < 0.0001 (when compared with vehicle).