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. 2015 Jan 22;144(2):382–392. doi: 10.1093/toxsci/kfv008

FIG. 3.

FIG. 3.

Effect of Thaps on the expression of HNF4α and C/EBPβ (LAP*, LAP, and LIP). A, Effect of Thaps on the expression of C/EBPβ (LAP*, LAP, and LIP) HepG2 cells were treated with Thaps at 50 nM for 12 or 24 h. Cells were collected, total RNA was isolated, and lysates were prepared. The level of C/EBPβ mRNA was determined by RT-qPCR (Left). Lysates (20 µg) were resolved by 7.5% SDS-PAGE and transferred electrophoretically to nitrocellulose membranes. The blots were incubated with a carboxylesterase antibody and developed with chemiluminescent substrate and re-probed by GAPDH antibody. The signal was captured by Carestream 2200 PRO Imager. Asterisk signs indicate statistical significance (P < 0.05). B, Effect of Thaps on the expression of HNF4α HepG2 cells was treated and samples were processed as described above. RT-qPCR was performed to determine the level of HNF4α mRNA whereas Western blotting was performed to determine the level of HNF4α protein. Asterisk signs indicate statistical significance (P < 0.05).