Skip to main content
. 2015 Jan 22;144(2):382–392. doi: 10.1093/toxsci/kfv008

FIG. 6.

FIG. 6.

Interplay of Thaps with antioxidants and rifampicin. A, Reversal of Thaps-mediated suppression of PXR by Z-guggulsterone HepG2 cells were treated Thaps at 50 nM, Z-guggulsterone (10µM), emodin (10µM), or in various combinations. The treatment lasted for 24 h and the expression of PXR mRNA was determined by RT-qPCR. The results were from 3 individual experiments in triplicate. Asterisk signs indicate statistical significance from the solvent control (P < 0.05). B, Effect of PXR transfection on the reduced induction of CYP3A4 by Thaps HepG2 cells were treated with rifampicin (10µM), Thaps (50 nM). Both transfected and non-transfected cells were used. The transfection was performed with a PXR expression construct or the corresponding vector. The treatment lasted for 24 h, cells were collected and total RNA was isolated. The level of CYP3A4 mRNA was determined. The results were expressed as fold of induction. Asterisk signs indicate statistical significance from nontransfected and RIF-treated cells (P < 0.05).