FIG 4.

HSP105 knockdown induces colorectal cancer cells to undergo apoptosis. (A) Viability of SW480 cells transfected with control or HSP105 siRNA for 144 h, as measured by Syto 60 nucleic acid staining. Viability was plotted relative to that of cells transfected with control siRNA. Error bars represent standard errors of the means of triplicate measurements. (B) SW480 cells were transfected with control (Ctrl) or HSP105 siRNA for 144 h. Cells were then collected and were stained with annexin V. (C) SW480 cells were transfected with control or HSP105 siRNA for 96 h, followed by transfection with a vector or Myc-ΔN-β-catenin for 48 h. Whole-cell lysates were immunoblotted with the indicated antibodies. (D) SW480 cells were transfected with control siRNA or with siRNA against HSP70 or CTNNB1 for 144 h. Whole-cell lysates were immunoblotted with the indicated antibodies.