TABLE 3.
MS analysisa of BRD4-immunoprecipitated histones from round spermatids
| Peptide and modification | Avg IP/total | SD |
|---|---|---|
| H3 9-17 | ||
| None | 1.02 | 0.18 |
| H3K9me1 | 0.65 | 0.28 |
| H3K9me2 | 1.26 | 0.73 |
| H3K9me3 | 4.24 | 1.51 |
| H3K9ac | 1.11 | 1.58 |
| H3K14ac | 1.09 | 0.08 |
| H3K9me1K14ac | 0.09 | 0.13 |
| H3K9me2K14ac | 1.35 | 1.09 |
| H3K9me3K14ac | 3.04 | 2.97 |
| H3K9,14ac | 0.00 | 0.00 |
| H4 4-17 | ||
| None | 1.14 | 0.20 |
| H4Kac1 | 0.61 | 0.12 |
| H4Kac2 | 0.98 | 0.08 |
| H4Kac3 | 3.72 | 1.04 |
| H4Kac4 | 10.07 | 5.17 |
| None | 1.17 | 0.21 |
| H4K5ac | 3.31 | 2.85 |
| H4K8ac | 2.67 | 4.89 |
| H4K12ac | 0.48 | 0.51 |
| H4K16ac | 0.87 | 0.42 |
| H4K5,8ac | 2.54 | 0.96 |
| H4K5,12ac | 1.36 | 0.87 |
| H4K5,16ac | 0.99 | 0.33 |
| H4K8,12ac | 0.83 | 0.35 |
| H4K8,16ac | 0.85 | 0.24 |
| H4K12,16ac | 0.80 | 0.13 |
| H4K5,8,12ac | 3.73 | 2.69 |
| H4K5,8,16ac | 4.18 | 1.51 |
| H4K5,12,16ac | 3.30 | 2.68 |
| H4K8,12,16ac | 2.82 | 1.54 |
| H4K5,8,12,16ac | 10.93 | 6.10 |
a
Quantification of the degrees of acetylation or methylation of histone H3 (amino acids 9 to 17, KSTGGKAPR) or H4 (amino acids 4 to 17, GKGGKGLGKGGAKR) peptide. Shown are average ratios of the percentage of H3 or H4 peptide in BRD4-immunoprecipitated chromatin to the total chromatin. Standard deviations were calculated for two and four biological replicates for H3 and H4, respectively.