TABLE 4.
MS analysisa of BRD4-immunoprecipitated histone H4 during spermatogenesis
| Cell type and modification | Total avg % | Total % SD | BRD4 IP avg % | BRD4 IP % SD |
|---|---|---|---|---|
| Sc | ||||
| None | 48.75 | 0.89 | 53.01 | 18.06 |
| 1ac | 39.41 | 1.37 | 27.42 | 2.80 |
| 2ac | 10.45 | 2.52 | 9.88 | 8.31 |
| 3ac | 0.94 | 0.15 | 3.60 | 2.42 |
| 4ac | 0.45 | 0.10 | 6.09 | 4.52 |
| RSp | ||||
| None | 30.39 | 0.46 | 37.40 | 4.57 |
| 1ac | 57.27 | 1.28 | 40.78 | 2.51 |
| 2ac | 8.67 | 1.13 | 7.70 | 0.49 |
| 3ac | 2.26 | 0.01 | 4.27 | 1.52 |
| 4ac | 1.42 | 0.32 | 9.85 | 3.08 |
| E/CSp | ||||
| None | 35.91 | 0.72 | 42.74 | 7.08 |
| 1ac | 34.90 | 3.29 | 35.69 | 3.77 |
| 2ac | 12.51 | 0.63 | 11.43 | 1.50 |
| 3ac | 8.55 | 0.76 | 5.78 | 1.22 |
| 4ac | 8.12 | 1.18 | 4.35 | 0.59 |
a
Quantification of the degrees of acetylation (% total peptide) of histone H4 peptide (amino acids 4 to 17, GKGGKGLGKGGAKR) in total and BRD4-immunoprecipitated chromatin from spermatocytes (Sc), round spermatids (RSp), and elongating/condensing spermatids (E/CSp). Standard deviations were calculated for two biological replicates.