FIG 5.

Roles of UPR in regulating polarity and growth in poor carbon sources. (A) Serial dilutions were spotted onto synthetic medium containing glucose or galactose. The ste12Δ mutant showed a modest growth defect on synthetic media with galactose. (B) Serial dilutions were spotted onto YEPD and YEP-GAL media. (C) UPRE-lacZ activity of the indicated strains in YEPD (GLU) and YEP-GAL (GAL). The experiment was performed in duplicate; error bars show standard deviations between strains. *, P < 0.05. (D) UPRE-lacZ activity was performed as described for panel C. The arrow refers to Msb2p overexpressed by the pGAL1 promoter. (E) Rhodamine-phalloidin staining of wild-type cells and the indicated mutants grown to saturation in YEPD medium. Tun, tunicamycin. Bar, 5 μm.