FIG 4.

The carboxyl-terminal domain of the native HJV uORF2-encoded peptide is inhibitory to the downstream main ORF translation. (A) Representation of the uORF2 construct encoding the native peptide, with the amino acid sequence shown below. Also, the amino acid sequences of the frameshift′2 to frameshift′16 constructs are shown, as well as the amino acid sequence of the peptide encoded by the uORF present in the frameshift uORF2 construct. These mutants were obtained from the parental uORF2 construct, in which the amino acid sequence of the uORF2-encoded peptide was successively altered by consecutive frameshift mutations, using the same strategy as that for the frameshift uORF2 construct shown in Fig. 3B. Amino acids are represented by letters; the altered amino acids are indicated in gray. (B) Relative translation activities expressed in HeLa and HepG2 cells for each of the constructs presented in panel A were obtained as described in the legend to Fig. 1B.