Groups (n= 5–6) of C57BL/6 or μmT mice were vaccinated intranasally with three doses of rCPAF+CpG (vaccinated) or treated with PBS (mock). One month following final vaccination, mice were challenged i.vag. with 5 × 104 IFU of C. muridarum. At day 80 after challenge, the mice were euthanized, genital tracts removed, embedded in paraffin, 5 μm sections made and stained with H&E. The H&E stained sections were observed under the microscope to score for the loss of uterine epithelial architecture. The individual scores for each uterine horn and the mean ± SE of all scores in a group are shown. *Significant differences between mock C57BL/6 and μmT mice (P = 0.005, Mann-Whitney Rank Sum test). ♣Significant differences between mock μmT mice and vaccinated C57BL/6 mice (P = 0.028, Mann-Whitney Rank Sum test). Results are representative of two independent experiments.