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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Exp Hematol. 2010 May 5;38(8):629–640.e1. doi: 10.1016/j.exphem.2010.04.004

Fig. 3. Expression of miR-23a cluster members in primary bone marrow hematopoietic cells.

Fig. 3

Taqman analysis (Applied Biosystems) of miR-23a, miR-27a, miR-24 expression in primary mouse hematopoietic populations. A) Lin- progenitors were prepared from bone marrow by depleting samples of cells expressing mature lineage markers. TER119+ erythroid cells, CD11b+GR1+ granulocytes and CD19+ B cells were isolated from bone marrow. For examination of miRNA expression in thymocytes, RNA was prepared from whole thymus. Expression was normalized to snoRNA 202 expression. Expression data is relative to Lin- cells. B) Bone marrow progenitors CMP, CLP, and GMP were isolated from mouse bone marrow. Expression of miRNAs is relative to expression in CMPs. MiR-21 expression was used as a control as its expression in these populations had been previously characterized[16].