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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Exp Hematol. 2010 May 5;38(8):629–640.e1. doi: 10.1016/j.exphem.2010.04.004

Fig 4. Expression of the miR-23a cluster in hematopoietic progenitors does not affect differentiation into monocytes and granulocytes.

Fig 4

Lin- cells were infected with MSCV or MSCV-23a27a24 retrovirus. Infected cells were then cultured 4 days in IMDM media containing the indicated cytokines A) Monocyte and granulocyte differentiation was evaluated by cell surface expression of F4/80 and Neut (7/4) on infected cells (GFP+). Neut+ fraction of cells contains granulocytes and the F4/80+ fraction contains monocytes B) No differences were detected in monocyte versus granulocyte differentiation between MSCV and miRNA expressing cells under any of the cytokine conditions tested. Percentages of cells in the monocyte and granulocyte gates are shown. C) MPRO cells (murine promyelocytic cell line) were infected with indicated retroviral plasmids. GFP+ (infected) cells were isolated, expanded, and lysed for RNA isolation. Taqman analysis was performed to quantify expression of the miR23a miRNAs. Expression is relative to MPRO cells infected with empty MSCV virus. Demonstrates that the cluster virus generates all three mature miRNAs.