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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Exp Hematol. 2010 May 5;38(8):629–640.e1. doi: 10.1016/j.exphem.2010.04.004

Fig 5. The 23a cluster promotes myeloid development of hematopoietic progenitors.

Fig 5

A) Lin- mouse hematopoietic progenitors were infected with empty viral vector, 23a cluster virus, or miR-21 virus. Cells were cultured on OP9 cells 12 days in presence of IL-7 and Flt3L. Differentiation analyzed by FACs by gating on GFP+ population and staining with fluorescently tagged antibodies to CD11b (myeloid), and CD19 (pro-B cells). Percentage of CD11b+, CD19+ and CD11b-CD19− cells is shown in top panel. . Lower panel denotes the actual number of events (cells) that were observed to be double negative, CD11b+, or CD19+. B) Average expression of CD19 and CD11b plotted from 4 independent OP9 co-culture experiments. Differences in the generation of myeloid and B cells were determined to be statistically significant by unpaired student-t-test. C) Cytospins of sorted GFP+CD11b+, and GFP+CD19+ cells from both MSCV and MSCV-23a27a24 cultures. Morphology indicates that CD11b, and CD19 expression is differentiating between myeloid and B cells.