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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Exp Hematol. 2010 May 5;38(8):629–640.e1. doi: 10.1016/j.exphem.2010.04.004

Fig 6. MiR-24 is necessary and sufficient to promote myeloid development of hematopoietic progenitors cultured on OP9 cells.

Fig 6

To determine if any single miRNA in the cluster was more responsible for the myeloid inducing activity we performed OP9 co-culture assays with cells infected with MSCV, 23a cluster, 23a only, 27a only, and 24 only expressing retroviruses. A) Infected Lin- progenitors were cultured on OP9 cells in the presence of Flt3L and IL-7 for 12 days. Differentiation was evaluated by cell surface expression of CD11b and CD19. (Cell percentages shown) B) Taqman analysis of RNA isolated from MPRO cells infected with MSCV viruses expressing a single cluster miRNA. Demonstrates that the indicated viruses produce the expected miRNAs in hematopoietic cells.