Fig 7. Expression of the 23a cluster in hematopoietic progenitor inhibits B cell development in vivo.
Marrow from 5-FU treated mice was infected with 23a cluster virus, and control MSCV virus. Cells were transplanted into irradiated mice and hematopoiesis examined by FACs between 6 and 7 weeks post-transplant. GFP positive and negative fractions were examined for hematopoietic development by cell surface markers. A) Bone marrow was examined for myeloid versus B lymphoid development by expression of CD11b, and CD19. B) Bone marrow B cell development was specifically examined by expression of CD19 and B220. C) Contribution to splenic B cells was examined by expression of B220 and IgM. Representative data is shown. Seven mice transplanted with MSCV-infected bone marrow and 9 mice transplanted with miR-23a cluster-infected bone marrow were examined.