TABLE 3.
Comparison of metabolites from cholate-grown culture supernatants of wild-type, ΔcasC, and ΔcasC/pTIPQcHiscasC R. jostii strainsa
| Compound | GC retention time (min) | m/z of parental ion | Relative abundance (%) |
||
|---|---|---|---|---|---|
| Wild type | ΔcasC/pTIPQcHiscasC complemented strain | ΔcasC mutant | |||
| THSBNC | 20.3 | 678 | 37 | 111 | 11.6 |
| HHIDP | 15.6 | 528 | 3 | 7 | ND |
| YHHIDP | 15.9 | 526 | 3 | 6 | ND |
| 2′-Pentanoate HHIDP | 17.9 | 556 | ND | ND | 128 |
a
Metabolites were derivatized with trimethylsilane and analyzed by GC-MS as described in Materials and Methods. The amount of each metabolite is presented relative to the added internal 5α-cholestane standard (0.25 mM; retention time on GC = 17.8 min). ND, not detected.