FIG 6.

Effect of introducing wild-type metK on the length of MNR2 cells. (A) PCR amplification of the metK gene, showing the restoration of the wild-type gene in SNR transductants. Lanes: 1, DNA ladder; 2, strain MEW1; 3 and 4, strain MEW649; 5, strain MNR2; 6, strain SNR. (B) Mean lengths of cells of strains MEW1, MEW649, MNR2, and SNR grown in minimal medium (dark shading) or LBNS (light shading) for 8 h. Error bars indicate standard deviations. (C) Distribution of lengths of SNR cells grown in either minimal medium (black curve) or LBNS (gray curve) for 8 h. The histogram shows the relative number of cells (as a percentage of all cells) with the indicated length in each culture. Cell length was measured using ImageJ with the ObjectJ plugin.