TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype and/or relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| MEW1 | E. coli K-12 ilvA Δlac | 8 |
| MEW649 | MEW1 ΔmetK pSAMT; Chlr | 10 |
| SM10 | Donor strain for mini-Tn10 mutagenesis | 28 |
| MNR2 | MEW649 ybdMN::Tn10kan; filaments in rich medium; Chlr | This study |
| MNR2A | MNR2 pSAMT-A; Chls Ampr | This study |
| SNR | MNR2 metK+; Chlr | This study |
| EC448 | E. coli Δ(λattL-lom)::bla lacIq P208-ftsZ-gfp | 7 |
| MNR2 λftsZ-gfp | MNR2 Δ(λattL-lom)::bla lacIq P208-ftsZ-gfp | This study |
| CAG12149 | dsbG601::Tn10; Tetr | 29 |
| Plasmids | ||
| pLtet01 | pLtet01 promoter, p15A replicon, MCS-1; Chlr | 8 |
| pSAMT | pLtet01 carrying the sam gene from Rickettsia prowazekii; Chlr | 10 |
| pSAMT-A | Ampr derivative of pSAMT | This study |
| pSC189 | Suicide vector for mini-Tn10 mutagenesis | 30 |
| pftsZ | ASKA plasmid carrying ftsZ under the control of an IPTG-inducible promoter; Chlr | 16 |
| pftsW | ASKA plasmid carrying ftsW under the control of an IPTG-inducible promoter; Chlr | 16 |
a
Chlr, chloramphenicol resistant; Ampr, ampicillin resistant; Tetr, tetracycline resistant.