FIG 7.

Quantitative Western blot for PilQ in PR100 and PR100 pilM^FS. (A) Representative quantitative Western blot showing the effect of the pilM frameshift mutation on the expression of PilQ multimers and monomers. Anti-PilQ antibody was detected with a Cy3-conjugated anti-rabbit secondary antibody using a Typhoon fluorescence imager. (B) The levels of PilQ multimers and monomers from 10 to 13 independent samples from 3 separate blots were quantified using ImageQuant software, as described in Materials and Methods. The mean decreases for the multimer and monomer in the pilM^FS mutant relative to the parental strain were 26% and 48%, respectively. *, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001. The error bars indicate standard errors of the mean.