FIG 8.

Gel filtration of PilQ extracted from FA19 membranes. FA19 membranes were extracted with 2% SB-10 and subjected to gel filtration on a Sephacryl S-500 column as described in Materials and Methods. The eluted fractions were assessed by SDS-PAGE and Western blotting for PilQ. The amounts of PilQ multimer and monomer were quantified using QuantityOne software. The arrows and molecular weights above the graph show the positions of protein standards. (A portion of this graph was reprinted from reference 23 with permission of the publisher.)