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Growth of auxotrophic liv mutant strains with different concentrations of ILV. Cells of strain BB3467 (liv) or BB3067 (bcaP braB brnQ liv) were grown overnight in TSS glucose-ammonium minimal medium with 200 μg of ILV/ml and then diluted 100-fold in the same medium containing various concentrations of ILV, as indicated. Each growth experiment was performed at least twice, and the results of a representative experiment are shown.
